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Biodiversity monitoring at the group scale is a important ingredient of assessing and finding out species distributions, ecology, variety, and movements, and it is essential to understanding and tracking environmental and anthropogenic results on natural ecosystems.1, 2, 3, 4 Vertebrates in terrestrial ecosystems are experiencing extinctions and declines in each inhabitants numbers and sizes on account of growing threats from human activities and environmental change.5, 6, 7, eight Terrestrial vertebrate monitoring utilizing existing methods is generally expensive and laborious, and although environmental DNA (eDNA) is becoming the tool of choice to evaluate biodiversity, few sample sorts successfully seize terrestrial vertebrate diversity. We hypothesized that eDNA captured from air could allow straightforward collection and characterization of terrestrial vertebrate communities. We filtered air at three localities within the Copenhagen Zoo: a stable, outdoors between the outside enclosures, and within the Rainforest House. Through metabarcoding of airborne eDNA, we detected forty nine vertebrate species spanning 26 orders and 37 households: 30 mammal, thirteen fowl, four fish, 1 amphibian, and 1 reptile species.
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